SubtiBank SubtiBank
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2017-8-11 16:0:32025-05-16 01:58:08

_ec

description

two-component sensor kinase, phosphorylates [[protein|Spo0F]], part of the [SW|phosphorelay], senses changes in respiratory activity

two-component sensor kinase, phosphorylates [[protein|Spo0F]], part of the [SW|phosphorelay]

locus

BSU31450

BSU_31450

outlinks

bsu

BSU31450

BSU_31450

The protein

Catalyzed reaction/ biological activity

autophosphorylation, phosphorylation of [[protein|Spo0F]]

mainly active in the older, inner regions of a colony (with [[protein|KinA]]) [Pubmed|21097618]

sensing potassium level in the medium during initiation of [SW|sliding] [Pubmed|26152584]

autophosphorylation, phosphorylation of [[protein|Spo0F]]

mainly active in the older, inner regions of a colony (with [[protein|KinA]]) [Pubmed|21097618]

senses potassium level in the medium during initiation of [SW|sliding] [Pubmed|26152584]

senses changes in respiratory activity [pubmed|23599347]

senses nutrient starvation in MM medium to initiate sporulation [pubmed|29314743]

ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

The protein

[SW|Domains]

six transmembrane segments, C-terminal histidine phosphotransferase domain

selectivity filter sequence of potassium channels that is required for [SW|sliding] but not for [SW|sporulation] [Pubmed|26152584]

six transmembrane segments, C-terminal histidine phosphotransferase domain

selectivity filter sequence of potassium channels that is required for [SW|sliding] but not for [SW|sporulation] [Pubmed|26152584]

[SW|Histidine kinase domain] (aa 218-426) (according to UniProt)

The protein

Structure

[PDB|3D36] (from G. stearothermophilus, complex with [[protein|Sda]])

[PDB|3D36] (from G. stearothermophilus, complex with [[protein|Sda]]) [pubmed|19101565]

Biological materials

Mutant

JH19980 (''kinB''::''tet'') [Pubmed|9334321]

JH19980 (''kinB''::''tet'') [Pubmed|9334321]

BKE31450 (Δ[[gene|kinB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGTGTGAAATCCTTTC, downstream forward: _UP4_TAGCAAATCGATTGGAACTT

BKK31450 (Δ[[gene|kinB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGTGTGAAATCCTTTC, downstream forward: _UP4_TAGCAAATCGATTGGAACTT

References

26152584, 8576055, 16166384, 9299348, 8497199, 10094672, 9426145, 11069677, 12618455, 11902725, 12618455, 7592498, 21097618, 19101565, 23378509, 23599347, 26152584, 28723971

26152584, 8576055, 16166384, 9299348, 8497199, 10094672, 9426145, 11069677, 12618455, 11902725, 12618455, 7592498, 21097618, 19101565, 23378509, 23599347, 26152584, 28723971, 29314743, 29321771, 19101565

The protein

Paralogous protein(s)

[[this]]