2017-8-11 16:0:32025-05-16 01:58:08
_ec
description
two-component sensor kinase, phosphorylates [[protein|Spo0F]], part of the [SW|phosphorelay], senses changes in respiratory activity
two-component sensor kinase, phosphorylates [[protein|Spo0F]], part of the [SW|phosphorelay]
locus
BSU31450
BSU_31450
outlinks
bsu
BSU31450
BSU_31450
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [[protein|Spo0F]]
mainly active in the older, inner regions of a colony (with [[protein|KinA]]) [Pubmed|21097618]
sensing potassium level in the medium during initiation of [SW|sliding] [Pubmed|26152584]
autophosphorylation, phosphorylation of [[protein|Spo0F]]
mainly active in the older, inner regions of a colony (with [[protein|KinA]]) [Pubmed|21097618]
senses potassium level in the medium during initiation of [SW|sliding] [Pubmed|26152584]
senses changes in respiratory activity [pubmed|23599347]
senses nutrient starvation in MM medium to initiate sporulation [pubmed|29314743]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
The protein
[SW|Domains]
six transmembrane segments, C-terminal histidine phosphotransferase domain
selectivity filter sequence of potassium channels that is required for [SW|sliding] but not for [SW|sporulation] [Pubmed|26152584]
six transmembrane segments, C-terminal histidine phosphotransferase domain
selectivity filter sequence of potassium channels that is required for [SW|sliding] but not for [SW|sporulation] [Pubmed|26152584]
[SW|Histidine kinase domain] (aa 218-426) (according to UniProt)
The protein
Structure
[PDB|3D36] (from G. stearothermophilus, complex with [[protein|Sda]])
[PDB|3D36] (from G. stearothermophilus, complex with [[protein|Sda]]) [pubmed|19101565]
Biological materials
Mutant
JH19980 (''kinB''::''tet'') [Pubmed|9334321]
JH19980 (''kinB''::''tet'') [Pubmed|9334321]
BKE31450 (Δ[[gene|kinB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGTGTGAAATCCTTTC, downstream forward: _UP4_TAGCAAATCGATTGGAACTT
BKK31450 (Δ[[gene|kinB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGTGTGAAATCCTTTC, downstream forward: _UP4_TAGCAAATCGATTGGAACTT
References
The protein
Paralogous protein(s)
[[this]]